16.3B: Transcriptional Enhancers and Repressors
Enhancers are cis-regulatory elements that enable precise spatiotemporal patterns of gene expression during development and are notable for being able Cited by: Enhancers are cis -regulatory elements that enable precise spatiotemporal patterns of gene expression during development and are notable for being able to function at large distances from their Cited by:
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New mutations of enhancer sequences and of the protein factors regulating enhancer function in human disease continue biooogy be identified, contributing to a growing class of 'enhanceropathies'. Katie Vicari. Banerji, J. Cell 27— Moreau, How to make pretzel dough without yeast. The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants.
Nucleic Acids Res. A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes. Cell 33— What are enhancers in biology, S. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.
Mercola, M. Transcriptional enhancer elements in the mouse immunoglobulin heavy chain locus. Science— Taub, R. Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. USA 79— Schroeder, M. How to make stripes: deciphering the wuat from non-periodic to periodic patterns in Drosophila segmentation.
Development— Levine, M. Transcriptional enhancers in biologu development and evolution. Klingler, M. Disperse versus compact elements for the regulation of runt stripes in Drosophila. Jack, J. Expression of the cut locus in the Drosophila wing margin is required for cell type specification and is regulated by a distant enhancer. Spana, C. The Drosophila melanogaster suppressor of Hairy-wing protein binds to specific sequences of the gypsy retrotransposon.
Genes Whzt. This study showed that Su Hw directly bound sequences in the gypsy insulator, thus providing a mechanistic understanding for years of genetic studies and helping to launch the new field of insulator biology.
Morcillo, P. Chip, a widely expressed chromosomal protein required for segmentation and activity of a remote wing margin enhancer in Drosophila.
Genes regulating the remote wing margin enhancer in the Drosophila cut locus. Genetics— This study used a genetic screen for factors regulating enhancer-promoter communication and identified genes later shown to encode the fly homologs of NIPBL and LDB1.
Rollins, R. Nipped-B, a Drosophila homologue of chromosomal adherins, participates in activation by remote enhancers in the cut and Ultrabithorax genes. Agulnick, A. Nature— Lee, S. Synchronization of neurogenesis and motor neuron specification by direct coupling of bHLH and homeodomain transcription factors. Neuron 38— Deng, W.
Controlling long-range genomic interactions at a native locus by targeted tethering of a looping factor. Cell— This study provided direct experimental confirmation that LDB1 bridges enhancer-promoter communication.
Torigoi, E. Chip interacts with diverse homeodomain proteins and potentiates bicoid activity in vivo. USA 97— Soler, E. The genome-wide dynamics of the binding of Ldb1 complexes during erythroid differentiation. Kagey, M. Mediator and cohesin connect gene expression and chromatin architecture. This study used genome-wide analyses and chromatin conformation assays to reveal the general requirement of cohesin in enhancer-promoter communication in mouse embryonic stem cells.
Schaaf, C. PLoS Genet. Bulger, M. Functional and mechanistic diversity of distal transcription enhancers. Kulaeva, O. Distant activation of transcription: mechanisms of enhancer action. Williams, T. World distribution, population genetics, and health burden of the hemoglobinopathies.
Cold Spring Harb. Kioussis, D. Van der Ploeg, L. Locus control regions: overcoming heterochromatin-induced gene inactivation in mammals. Fraser, P. Locus control regions, chromatin activation and transcription. Cell Biol. Gilbert, S. Google Enhancegs. Bonifer, C. Tissue specific and position independent expression of the complete gene domain for chicken lysozyme in transgenic mice.
EMBO Whay. Jones, B. The human growth hormone gene is regulated by a multicomponent locus control region. Kvon, E. HOT regions function as patterned developmental enhancers and have a distinct cis-regulatory signature. Selective inhibition of tumor oncogenes by disruption of super-enhancers. Whyte, W. Master transcription factors and mediator establish super-enhancers at key cell identity genes. Koch, F. Transcription initiation platforms and GTF recruitment at tissue-specific enhancers and promoters.
Parker, S. Chromatin stretch enhancer states drive cell-specific gene regulation and harbor human disease risk variants. USA— Hnisz, D. Super-enhancers in the what are enhancers in biology enhancera cell identity and disease.
Barolo, S. Shadow enhancers: frequently asked questions about distributed cis-regulatory information and enhancer redundancy. BioEssays 34— Lagha, M.
Enhancer locations In eukaryotic cell s the structure of the chromatin complex of DNA is folded in a way that function ally mimics the supercoil ed state character istic of prokaryotic DNA, so that although the enhancer DNA is far from the gene in regard to the number of nucleotide s, [>>>]. Sean Hasso, Joanne Chan, in Methods in Cell Biology, III Novel Chemical Strategies to Investigate Mechanisms of Angiogenesis. The zebrafish is a versatile vertebrate model organism amenable to both forward genetic and chemical library screens (Lieschke and Currie, ).In this section, we will discuss possible next steps in applying a chemical approach in the zebrafish model to.
Enhancer locations In eukaryotic cell s the structure of the chromatin complex of DNA is folded in a way that function ally mimics the supercoil ed state character istic of prokaryotic DNA, so that although the enhancer DNA is far from the gene in regard to the number of nucleotide s, Binding of specific proteins to an enhancer modulates the rate of transcription of the associated gene.
Figure Full glossary Enhancer A DNA sequence element to which transcriptional factors bind. Binding of transcriptional factors increases gene transcription. It may be located at various distances and in either orientation upstream from, downstream from or within a structural gene. The site binds cellular transcription factors , including steroid - receptor complexes. A base sequence that increases the rate of transcription of nearby genes ; the element need not be adjacent to the transcribed gene and enhancing activity is independent of orientation with respect to the gene.
Chemical mutagen , N-ethyl-N-nitrosourea. The construct includes a reporter gene fused to a minimal or weak promoter. A DNA sequence that transcription factors known as activators can recognize and bind to and thereby aid in recruiting RNA polymerase to a gene's promoter to increase transcription.
They are located 10 to 50 kb downstream or upstream of a gene. They may be tissue-specific. May be several thousand base -pairs away from the promoter. PMC PMID The foreign protein then has to be purified from all of the insect cells or the bacteria l cells. Topic: Biology. Home » Biology » Enhancer. Enhancer Enhancer locations In eukaryotic cell s the structure of the chromatin complex of DNA is folded in a way that function ally mimics the supercoil ed state character istic of prokaryotic DNA, so that although the enhancer DNA is far from the gene in regard to the number of nucleotide s,